2. Methods

C.    Description in detail of method or procedures (The following are important and key items that should be included when formulating ANY AND ALL research plans.)

Equipment list:
  • Lemongrass seeds ( 18 seeds )
  • Light lamps (2-4)
  • UVB light lamps ( 1-2 )
  • Boxes/ Containers (18)
  • A bag of soil (100cm^3)
  • Water (800ml)

Procedures: Detail all procedures and experimental design to be used for data collection

  1. First prepare 8 identical containers ( In this case, we used plastic party cups.)
  2. Fill each container with soil and add 50ml of water to each container to make the soil moist.
  3. Place 3 seeds into each container.
  4. Attach artificial lighting (White LEDs) on the roof of Cardboard box.
  5. Attach a UV light source next to the artificial light
  6. Change the dependent variable: Presence of UV light.
  7. Do 8 set-ups (6 groups of 3 set-ups and 2 control experiments.)
  • Three seeds (1 set-up) grown in artificial light and UV light for 30 minutes before turning UV light off and letting them grow for 42 hours under artificial light.
  • Three seeds (1 set-up) grown in artificial light and UV light for 60 minutes before turning UV light off and letting them grow for 42 hours under artificial light.
  • Three seeds (1 set-up) grown in artificial light and UV light for 90 minutes before turning UV light off and letting them grow for 42 hours under artificial light.
  • Three seeds (1 set-up) grown in artificial light and UV light for 120 minutes before turning UV light off and letting them grow for 42 hours under artificial light
  • Three seeds (1 set-up) grown in artificial light and UV light for 150 minutes before turning UV light off and letting them grow for 42 hours under artificial light.
  • Three seeds (1 set-up) grown in artificial light and UV light for 180 minutes before turning UV light off and letting them grow for 42 hours under artificial light.



10. Then measure the height of the plants in the containers and record it down.
11. Compare height with controls.
12.Take the average height of the plants in each group of three seeds (1 set-up).
13. Then check if Hypotheses if it is correct.
14. Draw Conclusion



• Risk and Safety: Identify any potential risks and safety precautions to be taken.
  • Take caution when attaching the different equipment such as the diodes and water droppers so that they do not fall into the experiment.
  • We might get over-exposure to UV Light. Minimise exposure to UV Light as much as possible by covering it the set-ups with boxes.
  • The plants may be contaminated. We should use gloves to handle the plants after exposure to UV Light.

Data Analysis: Describe the procedures you will use to analyze the data/results that answer research questions or hypotheses
  1. Measure the average height of the plants per time exposed to UV light by measuring the height of each of the plants in one group of set-ups and drawing an average
  2. Draw a graph with the time exposed to plants the x-scale and the average height of the plants the y-scale.

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